Open Access Conference Proceedings

Multiresistant bacterial strains are a very serious clinical problem in a hospital environment. Solving these types of nosocomial infections requires new and unique approaches. It is known that vancomycin is an antibiotic used for the treatment of serious infections such as S. aureus strains. Nanomedicine using new nanotechnologies brings new options like application of surface coating modifications for the targeting treatment. Superparamagnetic iron oxide nanoparticles (SPION) particles were created by a physicochemical method of synthesis. For the purpose of this testing, SPION particles have been modified by chitosan (300 rpm, 25 ℃, 24 h), then purified on the magnet. SPION/Chito particles were modified by vancomycin (300 rpm, 25 ℃, 6 h). The completed particles were washed with a PBS buffer (pH 7). The concentration of vancomycin (after the modifications) in SPION/Chito/VANCO particles was 100 µg/ml. The stability of finalized particles was tested in PBS (pH 7). The concentration of vancomycin was determined by HPLC. After that, the antibacterial activity was determined by measurement of growth curves (methicillin sensitive and resistant S. aureus) at 450 nm/540 nm for 24 hours (25 °C, 300 rpm in LB medium). The results were evaluated as AUC growth curves. Bacterial cultures were grown reproducibly in pure medium (AUCs were considered baseline). The study provided initial information about the effect of newly prepared types of nanoparticles on S. aureus species.

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