from the conferences organized by TANGER Ltd.
Over the last decade, drug delivery systems for wound management have undergone a significant evolution as novel nano-based dosage forms and wound dressing pads for active wound management have been developed. Significance of these materials is based on increasing demand for active wound dressing able to battle infection and inflammation in contaminated and slow-to-heal wounds. Nanofibres represent nanomaterial able to act simultaneously as wound dressing protecting the wound and drug delivery system for in situ drug release. Bioavailability of the drug and its release kinetics strongly depend on the drug nature, nanofibrous matrix characteristics and mechanism of drug incorporation. In bacterial infection treatment, fast reaching of minimal inhibitory concentration (MIC) is crucial and can be achieved by mediated surface drug adsorption. In this work, we explored interactions of electrospun silica nanofibres with five model antibiotics from tetracycline family (tetracycline, tetracycline hydrochloride, oxytetracycline hydrochloride, chlortetracycline hydrochloride and doxycycline hydrochloride) in order to evaluate robustness of this novel nanofibrous drug delivery system. The drug adsorption was mediated by surface functionalization with amino groups. Drug functionalized nanofibres were studied in terms of morphology and total drug content. Antibacterial activity against bacteria E. coli was tested in vitro. Overcoming the MIC value was achieved for all tested antibiotics. Biocompatibility was tested and confirmed on human dermal fibroblasts in vitro. Presence of all tested antibiotics led to increased cellular metabolic activity. Based on these results we assumed that silica nanofibres represent a robust system able to bind and release variety of tetracycline antibiotics for contaminated wounds management.
Keywords: Silica, nanofibres, drug delivery, antibiotics, wound healing© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.