Open Access Conference Proceedings

Tissue engineering and regenerative medicine are ever-growing multidisciplinary fields. Along with the development of tissue carriers, methods for testing the biocompatibility of these materials are also being developed. This work deals with research, development, and optimisation of the preparation of a fibre system intended for monitoring the direct interaction of the 3T3 mouse fibroblast cell line with polymeric materials in real-time (live cell imaging). The fabricated microfiber model (grid) was seeded with the 3T3 mouse fibroblast cell line, and the interaction between the materials and the cells was monitored for three days by optical microscope. The interaction between the materials and the cells was observed during a picture of cells on the fibres. The advantage of this method is the absence of other chemicals like chemicals for the visualisation of cells (fluorescence staining) or chemicals for measuring cell viability because cells adhering to fibres are visible in transmitted light.

Keywords: Microfibers, drawing, grid model system, cell interaction

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