from the conferences organized by TANGER Ltd.
One of the many interesting properties of silver nanoparticles is their antimicrobial activity. Since these properties are related to the morphology of the particles, it is interesting to pay attention to the antimicrobial effects of various metal nanostructures, which differ in the method of synthesis, size, or shape. One of the very interesting methods of synthesis of metal nanoparticles, which seems to be very advantageous because of its low cost, efficiency, and ecology, is the synthesis using plant extracts. For this work, woody parts of the Vitis vinifera were selected as material for the preparation of the extract. The canes of this plant are an agricultural waste containing many bioactive substances, which are not used for other significant purposes, making it a promising material for the preparation of metal nanoparticles with antimicrobial potential. The biosynthesized nanoparticles were detected and characterized using UVVis spectrophotometry, transmission electron microscopy (TEM) and dynamic light scattering (DLS). TEM showed that the obtained nanoparticles have a heterogeneous shape with a size 4–51 nm. The DLS analysis supported that the Z-average was 113.8 nm and 0.165 PDI value. The use of biosynthesized nanoparticles showed an excellent growth inhibition property against two strains of the opportunistic pathogenic bacteria Pseudomonas aeruginosa (ATCC 10145 and ATCC 15442). Furthermore, a bactericidal concentration (BC) 40% v/v of AgNPs was found for P. aeruginosa ATCC 15442, while no BC was found in the range of tested concentrations for P. aeruginosa ATCC 10145.
Keywords: Biosynthesis, plant extract, nanoparticles, antimicrobial activity© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.