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In this work, two-dimensional layered material Ti3C2TX MXene has been used to immobilize enzyme sarcosine oxidase to fabricate a nanostructured biosensor. The device was applied for detection of sarcosine, a potential prostate cancer biomarker, in urine samples for the first time. The morphology and structures of MXene have been characterised by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Electrochemical, SEM and AFM results revealed that MXene along with chitosan is a good candidate for enzyme immobilization to fabricate a sensitive nanobiosensor. The prepared device exhibited a good analytical performance with a low detection limit of 18 nM and linear range from 0.09 to 7.8 µM. The proposed biosensing method also demonstrated high recovery index for sarcosine detection in urine samples in clinically relevant range (102.6 %).
Keywords: MXene, sarcosine, biosensor, prostate cancer© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.