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This study presents the development of the lateral flow assay (LFIA) for the determination of undeclared chicken additives in meat products. In the test system, immunoglobulins were proposed as biomarkers for species identification. A sandwich format of the LFIA based on gold nanoparticles (AuNPs) as nano-dispersed labels and colorimetric detection (both visual and instrumental) is used to determine immunoglobulins and, consequently, a source of meat. To achieve the highest analytical parameters, the LFIA was optimized by varying the assay conditions. A simple method of sample preparation was proposed. The developed LFIA allows distinguishing poultry (chicken, turkey) meat sources from mammalian sources within 20 min. The developed LFIA was applied to analyze meat foodstuffs and allowed revealing an adulteration with up to 0.063% (w/w) sensitivity. The proposed test system can be recommended for rapid on-site screening control of the composition and quality of meat products.
Keywords: Immunoglobulins, lateral flow immunoassay, meat adulteration, chicken additives© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.