TEST OF COMPLEX NANOSTRUCTURES AS GENE DELIVERY SYSTEM TO CARDIAC CELLS

1,2 SKOPALIK Josef
Co-authors:
3,4 PRUCHA Jaroslav 2 SEKORA Jiri 2 CMIEL Vratislav 1 PARAK Tomas 5,6 MASEK Josef
Institutions:
1 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic, EU, j.skopalik@gmail.com
2 Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, Czech Republic, EU
3 Department of Health Care Disciplines and Population Protection, Faculty of Biomedical
4 Department of Information and Communication Technologies in Medicine, Faculty of Biomedical Engineering, Czech Technical University, Prague, Czech Republic, EU
5 Centre of Experimental Medicine, Slovak Academy of Sciences, Bratislava, Slovakia, EU
6 Department of Pharmacology and Toxicology, Veterinary Research Institute, Brno, Czech Republic, EU
Conference:
12th International Conference on Nanomaterials - Research & Application, Brno, Czech Republic, EU, October 21 - 23, 2020
Proceedings:
Proceedings 12th International Conference on Nanomaterials - Research & Application
Pages:
342-346
ISBN:
978-80-87294-98-7
ISSN:
2694-930X
Published:
28th December 2020
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
700 views / 405 downloads
Abstract

Various nanostructures, including liposomes, dendrimers, polymers, carbon or silicon materials, and metal nanoparticles seems to be evaluated as carriers in different gene delivery systems. Muscle cells are target of modern gene medicine, due to of the much pathology (arrhythmias, channels pathological mutation, abnormal apoptosis) with limited curability by traditional drugs. The zeta potential of the muscle cells were identified by non-traditional method of DLS. This was starting point to setting of parameters for experimental liposome, the liposome surface charge have been tested and modified by cationic plasmid and final complex was evaluated as delivery system of DNA into the muscle cardiac cells.

Keywords: Nanoliposom, nanoparticle, transfection, zeta potential, cell engineering, regenerative medicine

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