SPECIAL NANOFIBER SCAFFOLD DESIGNED FOR CELLULAR PRODUCTS IN THE TREATMENT OF CHRONIC WOUNDS

1 Braťka Petr
Co-authors:
2 Buzgo Matej 3 Walaská Hana 4 Rosina Josef 1 Červeňák Martin 1 Louda Matěj
Institutions:
1 Grade Medical s.r.o., Řevnice, Czech Republic, EU
2 InoCure s.r.o., Čelákovice, Czech Republic, EU
3 ING MEDICAL s.r.o., Prague, Czech Republic, EU
4 Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic, EU
Conference:
12th International Conference on Nanomaterials - Research & Application, Brno, Czech Republic, EU, October 21 - 23, 2020
Proceedings:
Proceedings 12th International Conference on Nanomaterials - Research & Application
Pages:
335-341
ISBN:
978-80-87294-98-7
ISSN:
2694-930X
Published:
28th December 2020
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
795 views / 503 downloads
Abstract

In this work we have prepared and characterized several types of nanofibers to create unique nanofiber substrates as a medical device for long-term non-healing wound treating using cellular products. Our nanofibers are based on biodegradable polymers such as polycaprolactone (PCL), poly(lactide-co-caprolactone) (PLCL), poly(vinyl)alcohol (PVA), polyethyleneimine (PEI) and hyaluronic acid. Prepared nanofiber carriers were specifically adjusted for the autologous MSCs (mesenchymal stromal cells) applications on the chronic wound. In addition, the nanofiber sheets were constructed as a biodegradable material to guarantee proper timing of its resorption in a chronic wound during the healing process. Furthermore, the effect of growth factors on MSC proliferation was tested. The release of bFGF from adsorbed lipid particles was tested. The results show increased proliferation of MSCs. Despite fast increasing advanced technologies in healthcare, chronic wound treating tends to be still very difficult and challenging. Our developed nanofiber-based dressings are very promising for poorly healing wound therapy using MSCs. The main goal is to make chronic wound healing more effective and decrease a time of healing process.

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