PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS – ANTIBACTERIAL EFFECT

1,2 RUTTKAY-NEDECKÝ Branislav
Co-authors:
1,3,4 SEHNAL Karel 5 BANAS Dominik 1,3 STANKOVA Martina 6 KEPINSKA Marta 7 BACH Duong Ngonc 1 SOCHOR Jiri 1 BARON Mojmir 8 FARID Awais 7 NGUYEN Hoai Viet 1,3,4,6 KIZEK René
Institutions:
1 Faculty of Horticulture, Department of Viticulture and Enology, Mendel University in Brno, Lednice, Czech Republic, EU, karelsehnal15@gmail.com, jiri.sochor@mendelu.cz
2 Department of Molecular Pharmacy, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic, EU, ruttkay-nedeckyb@pharm.muni.cz
3 Department of Research and Development, ECO-ENVI-NANOLIFE, Prague, Czech Republic, EU
4 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic, EU, kizek@sci.muni.cz
5 Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice, Brno-Bohunice, Czech Republic, EU, dominik.banass@gmail.com
6 Department of Biomedical and Enviromental Analyses, Faculty of Pharmacy, Wroclaw Medical University, Wroclaw, Poland, EU marta.kepinska@umed.wroc.p l
7 Research Center for Environmental Monitoring and Modeling, VNU University of Science, Hanoi, Vietnam, nguyenviethoai@hus.edu.vn
8 Divison of Environment and Sustainability, Hong Kong University of Science and Technology, Kowloon, Hong Kong, awais@ust.hk
Conference:
12th International Conference on Nanomaterials - Research & Application, Brno, Czech Republic, EU, October 21 - 23, 2020
Proceedings:
Proceedings 12th International Conference on Nanomaterials - Research & Application
Pages:
422-427
ISBN:
978-80-87294-98-7
ISSN:
2694-930X
Published:
28th December 2020
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
862 views / 502 downloads
Abstract

Several silver nanoparticles (AgNPs) with biological effect were prepared by green synthesis. AgNPs were prepared from Vitis vinifera seeds (Merlos variety) used as wine production waste. Green synthesis brings intensive surface modifications of nanoparticles. The seeds were lyophilized and then homogeneously ground. Purified extracts (0.5, 2.5, 5 and 10 g / 100 mL water) were added to AgNO3 solution(1 M) in a 1:1(v/v) ratio and stirred for 24 h. The formed nanoparticles were precipitated with methanol (1:1) (v/v) and lyophilized. Secondary metabolites were analyzed by various methods. The preparation yield of AgNPs ranged between 3 - 25 %. The total protein values determined by the Lowry method ranged between 1.8–3.6 mg/mL. Antioxidant activity values determined by the CUPRAC method ranged between 76 – 157 µg/mL GAE. Total phenols values determined by Folin-Ciocalteu ranged between 88 – 160 µg/mL GAE, by 4-aminoantipyrine method (TAAP) ranged between 728 - 1,299 µg/mL GAE, by Price Butler method (PBM) ranged between 57 - 108 µg / mL GAE. AgNPs prepared from Vitis vinifera seeds (AgNPs-VV) showed also antibacterial activity. Minimal inhibition concentration (MIC) of AgNPs-VV in S.aureus was 31 µg/mL and MIC in E. coli was 55 µg/mL.

Keywords: Green synthesis, phenolic compounds, analytical methods, antibacterial effects; traditional medicine, ethnobotany

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