GOLD NANOPARTICLES MODIFIED SCREEN PRINTED CARBON ELECTRODE AS A TOOL FOR DETECTION OF TP53

1 KOUDELKOVÁ Zuzana
Co-authors:
1,2 SEDLÁČKOVÁ Eliška 1,2 Richtera Lukáš 1 ŠVEC Pavel 1,2 ADAM Vojtěch
Institutions:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-61300 Brno, Czech Republic
2 Central European Institute of Technology, Brno University of Technology, Purkyňova 123, CZ-61200 Brno, Czech Republic
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
402-405
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
1025 views / 467 downloads
Abstract

Cancer is one of the most common causes of death in developing countries and in the developed world. The function of the p53 protein plays an important role in the development of cancer. The protein p53 regulates the expression of genes that are responsible for the control of cell growth, apoptosis or for repair of damaged DNA. It t is encoded by the tumor suppressor gene TP53. If this gene mutates, its product will be damaged. It was found that mutations in the TP53 gene is present in more than 50% of cancer cases. Electrochemical biosensors have proved to be a suitable method for the detection of TP53 and also its mutations. Electrochemical deposition of gold nanoparticles on the surface of working electrode increases the active surface area of the sensor, this leads to a more sensitive detection of the TP53 gene and its mutations. By developing an electrochemical biosensor based on gold nanoparticles modified screen printed electrodes, an efficient platform for detecting the TP53 gene in biological samples is obtained.

Keywords: Screen printed electrode, gold nanoparticles, gene TP53, protein p53, electrochemistry

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