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MAGHEMITE PARTICLES FOR SPERMIDINE SEPARATION

1,2 KOPEL Pavel
Co-authors:
1,2 CERNEI Natalia 3 HORKY Pavel 1,2 LACKOVA Zuzana 1,2 MILOSAVLJEVIC Vedran 1,2 GAGIC Milica 1,2 ZITKA Ondrej 1,2 ADAM Vojtech
Institutions:
1 Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, EU, vojtech.adam@mendelu.cz
2 Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic, EU
3 Department of Animal Nutrition and Forage Production, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, EU
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
353-357
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
DOI:
https://doi.org/10.37904/nanocon.2019.8496
Published:
1st April 2020
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
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Abstract

We report the optimal conditions for the separation of spermidine from different types of samples, for example blood or cancer cells and its subsequent determination by ion-exchange liquid chromatography (IEC) with UV-VIS detector. Here in, we synthesized paramagnetic particles able to isolate and immobilize spermidine from blood of patients with cancer or cancer cells and thus preconcentrate it for analysis. Dowex surface was covered by nanomaghemite (γ-Fe2O3) or maghemite particles surface was modified by chitosan and sulfoxyethyl cellulose. The best separation properties showed Dowex microparticles. The paramagnetic particles can be used in the future for isolation of spermidine from real samples and diagnosis of cancer.

Keywords: Ion exchange chromatography, maghemite, spermidine, chitosan and sulfoxyethyl cellulose

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