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COMPARISON OF PDMS MICROTOPOGRAPHICAL PLATES AND PLANAR NANOFIBER SCAFFOLDS FOR IN VITRO CULTIVATION OF CARDIAC CELLS

1 SKOPALIK Josef
Co-authors:
2 SLAVIK Jan 1 CMIEL Vratislav 4 POLAKOVA Katerina 3 PRUCHA Jaroslav 4 GABRIELOVA Eva 1 BAIAZITOVA Larisa 1,2 SVOBODA Ondrej 1 PROVAZNIK Ivo
Institutions:
1 Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, Czech Republic, j.skopalik@gmail.com
2 Central Institute of Technology (CEITEC), Brno University of Technology, Brno, Czech Republic
3 Department of Health Care Disciplines and Population Protection, Faculty of Biomedical Engineering, Czech Technical University in Prague, Prague, Czech Republic
4 Department of Medical Chemistry and Biochemistry, Faculty of Medicine, Palacky University; Olomouc, Czech Republic
5 Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Experimental Physics and Analytical Chemistry, Faculty of Science Palacky University Olomouc, Czech Republic
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
442-447
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
526 views / 228 downloads
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Abstract

PDMS microchambers and microchannels were evaluated in many studies of the specific in vitro cultivation. Modern microtopographical methods bring new possibilities how to prepare transparent planar blocks with isotropic lamellas. On the other hand, the planar nanofiber sheets are another type of modern culture substrate. Neonatal myocytes and the cardiac muscle cell lineage HL-1 were used for tests of the cell adherence, cell survival and cell orientation on pure or modified polydimethylsiloxane (PDMS) or polycaprolactone (PCL). PDMS with fibronectin coating was shown to be the material with the best cell adherence, viability and isotropic cell orientation from all tested variants.

Keywords: Nanofibers, microlithographical methods, in vitro models, tissue engineering, cardiomyocytes

© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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