ELECTROSPINNING AND BIOCOMPATIBILITY OF POLYMER-CERAMIC NANOFIBERS FOR TISSUE ENGINEERING

1 ŠŤASTNÁ Eva
Co-authors:
1 ČÁSTKOVÁ Klára
Institution:
1 Ceitec - Central European Institute of Technology, Brno, Czech Republic, EU, Eva.Jindrova@ceitec.vutbr.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
472-476
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
658 views / 183 downloads
Abstract

Polycaprolactone is known as biocompatible material for long time and it is considered as possible material for bioscaffolds. Since natural structure of hard tissues (bones) and most of soft tissues (skin, neural tissue) is fibrous, there are attempts to mimic such a structure. Polycaprolactone (PCL) nanofibers with diameter of approximately 180 nm were prepared by electrospinning method. For better biocompatibility and bioactivity, a composite fibrous structure was prepared. Hydroxyapatite nanoparticles were synthetized via precipitation reaction and further hydrothermal treatment. The ceramic particles were added to modified polycaprolactone precursor and the solution was electrospun. The prepared fibers and as well as dried original precursor were in-vitro tested for cytotoxicity by direct contact test. Mouse fibroblast from L929 line were used for cell cultivation. It was found out that fibrous structure and presence of ceramic particles have a positive influence on cell activity and proliferation – the growth rate of the cells was significantly higher compared to bulk polymer precursor.

Keywords: Nanofibers, electrospinning, biocompatibility, polycaprolactone

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