Open Access Conference Proceedings

Surface functionalization of 2D MoS2 materials offers a convenient way to tune their properties and facilitate their application in the field of bio-sensing and nanomedicine. In this study, three different liquid phase exfoliation (LPE) methods were utilized for MoS2 exfoliation: LPE in 45% ethanol solution, LPE in ultrapure water and amino acid intercalation assisted LPE. The three products were bio-conjugated with PEG molecules and through a biotin-avidin-biotin bridge they were functionalized with specific antibody M75 having exclusive affinity towards cancer cells expressing CAIX protein on their membrane. We are aiming to achieve high selectivity towards cancerous cells utilizing an antigen-antibody based cancer detection scheme. This study uses CAIX antigen tagged MDCK cells simulating the cancerous environment and pure MDCK cells as control. Cellular intake studies were performed by confocal Raman microscopy imaging and confocal laser scanning microscopy before and after binding the specific antibody. The preliminary results indicate higher density of internalized nanoplatforms in the case of MDCK cells expressing CAIX compared to the control cells. However, further studies are required to validate these results.

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