PRIMARY EVALUATION OF PCL/PEI NANOFIBRES IN TERMS OF THE SURFACE FUNCTIONALITY FOR BIOMOLECULES ATTACHMENT

1,2 RYSOVÁ Miroslava
Co-authors:
1 TOMÁNKOVÁ Hana 1,2 VAŇÁTKOVÁ Petra
Institutions:
1 Institute for Nanomaterials, Novel Technologies and Innovation, TUL, Liberec, CZ
2 Institute of New Technologies and Applied Informatics, FM, TUL, Liberec, CZ, *miroslava.rysova@tul.cz
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
605-610
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
500 views / 326 downloads
Abstract

Nanofibrous matrices represent an attractive material for biomedical applications – mainly due to their high specific surface and small pore size, which make them interesting material for drug delivery matrixes construction. Besides the morphological characteristics, the surface functionality strongly affects the drug-matrix interaction, drug conjugation and drug release kinetics. Implication of primary amino (-NH2) groups to the surface of nanofibres can improve their performance and enable conjugation of drug via covalent binding and/or electrostatic interactions. The aim of this study was to evaluate applicability a nanofibrous material based on blend of two biocompatible and biodegradable polymers – poly-Ɛ-caprolactone (PCL) and polyethylenimine (PEI) produced by needleless electrospinning. The electrospun matrices were evaluated in terms of morphology, functional groups availability and short term stability. Moreover, biocompatibility to the 3T3 fibroblastic cell line was verified in direct contact. Binding capacity estimation was performed on a model molecule (BSA) in order to demonstrate binding capacity of the system and its applicability for protein immobilization for wound management.

Keywords: nanofibres, polycaprolactone (PCL), polyethylenimine (PEI)

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