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ISOLATION OF REDUCED AND OXIDIZED GLUTATHIONE USING MAGHEMITE PARTICLES

1,2 CERNEI Natalia
Co-authors:
1,2 KOPEL Pavel 1,2 MILOSAVLJEVIC Vedran 3 HORKY Pavel 1,2 ADAM Vojtech
Institutions:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
2 Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic
3 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
165-169
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
529 views / 276 downloads
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Abstract

Here we report the optimal conditions for the isolation of reduced glutathione (GSH) and oxidized glutathione (GSSG) from various types of matrix, such as blood, plasma or cells and their characterization using ion-exchange liquid chromatography with VIS detector. In our study, we aimed to synthesize paramagnetic microparticles able to isolate and immobilize GSH, GSSG from blood or plasma and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified amberlite and Dowex by nanomaghemite (γ-Fe2O3), as the functional carriers providing perfect paramagnetic properties. Both the paramagnetic microparticles were able to bind GSH and GSSG. Our paramagnetic microparticles have potential to improve GSH and GSSG isolation and in future can serve for application as a platform of biosensors or delivery system.

Keywords: Ion Exchange Chromatography; Maghemite Microparticles; glutathione; reactive oxygen species

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