USE OF GD-DOPED ZNO SURFACES TO INCREASE FLUORESCENCE OF GLOBULAR PROTEINS

1 HAYRULLINA Indira
Co-authors:
1,2 BUTUSOV Leonid 2,3,4 NAGOVITSYN Ilia 2,3 CHUDINOVA Galina 1 SHULGA Aleksandra 1 KURILKIN Vladimir 1 KOCHNEVA Margarita
Institutions:
1 RUDN University - Peoples’ Friendship University of Russia, Moscow, Russia
2 Natural Science Center of General Physics Institute RAS, Moscow, Russia
3 National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, Russia
4 Semenov Institute of Chemical Physics RAS, Moscow, Russia
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
161-164
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
618 views / 348 downloads
Abstract

Novel ZnO – Gd3+ materials were prepared by sol-gel method and photoluminescence properties of their films were studied for biomedical application. Biosensor’s properties ZnO – Gd3+ films were investigated under the action of rabbit immunoglobulin G (IgG) in concentrations 10-9, 10-11 and 10-13 M in solution depositing on the surface of film. The doping of ZnO film by Gd3+ in an amount from 1-12 mass.% with increments of 1 mass. % results in significant variations of fluorescence spectra ZnO – Gd3+ films. The ZnO substrate without of Gd3+ compounds allows detecting IgG in a concentration of 10-11 M by fluorescence spectroscopy method by registration in UV region (320-450 nm). By using ZnO – Gd3+ system it was obtained the increase the concentration limit of immunoglobulin (10-13M) detection. In the only case (the content of Gd3+ in ZnO film - 8 mass. %) was observed batochromic shift of fluorescence band maximum by 20 nm (from 364 to 385nm) when interacting with immunoglobulin regardless of the concentration of the last.

Keywords: ZnO, gadolinium, sol-gel method, photoluminescence, fluorescence, immunoglobulin G, IgG, biosensor

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