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CHARACTERISATION OF PEROXIDASE-LIKE ACTIVITY OF THERMALLY SYNTHESIZED GOLD NANOPARTICLES

1 DOCEKALOVA Michaela
Co-authors:
1 UHLIROVA Dagmar 1 STANKOVA Martina 2 KEPINSKA Marta 3 SOCHOR Jiri 2 MILNEROWICZ Halina 1 BABULA Petr 4 FERNANDEZ Carlos 1 BRAZDOVA Marie 5 ZIDKOVA Jarmila 1 SUCHY Pavel 1,2 KIZEK Rene
Institutions:
1 University of Veterinary and Pharmaceutical Sciences Brno, Pharmaceutical Faculty, Czech Republic, EU
2 Wroclaw Medical University, Wroclaw, Poland, EU
3 Mendel University in Brno, Faculty of Horticulture, Lednice, Czech Republic, EU
4 Robert Gordon University, School of Pharmacy and Life Sciences, Aberdeen, United Kingdom, EU
5 Institute of Chemical Technology, Prague, Czech Republic, EU
Conference:
8th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 19th - 21st 2016
Proceedings:
Proceedings 8th International Conference on Nanomaterials - Research & Application
Pages:
429-434
ISBN:
978-80-87294-71-0
ISSN:
2694-930X
Published:
17th March 2017
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
551 views / 331 downloads
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Abstract

The development of nanotechnologies has attracted a lot of interest since it brings new possibilities in the field of nanomedical applications, from diagnostic methods to targeted nanotransporters. Gold nanoparticles (AuNPs) belong to a group of nanomaterials with significant peroxidase-like activity. We have successfully synthesized AuNPs thermally over a range of temperatures from 20 to 100 °C. The AuNPs size varied from 17 to 37 nm and the zeta potential was found to vary from -32 to -44 mV, in which the maximum absorption maximum was detected at 529 nm. Peroxidase-like activity was monitored in the presence of TMB (1 mM) and hydrogen peroxide (10 mM). The proposed system based on peroxidase-like activity was further evaluated in immune detection of human IgG, in which anti-IgG antibody was modified with AuNPs.

Keywords: Gold nanoparticles, electrochemistry, spectrophotometry, enzymatic reaction, ELISA, nanomedicine

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