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Nanofibers are valued for their ultra-high specific surface areas and have been found potentially useful in many biomedical applications. Modification or functionalization of nanofibers is necessary in order to engineer specific features that will help to maximize their end-use performance. A spectrum of bioactive molecules, including antibacterial agents, anti-cancer drugs, enzymes, proteins, can be incorporated into/onto nanofibers via different approaches [1].We have focused on enzyme immobilization providing many advantages over use of its soluble form: enhanced enzyme activity, reusability, simplified processing, better storage properties and pH stability [2].Here we describe the immobilization of trypsin (EC 3.4.21.4) on silica nanofibers. After silanisation incorporating 72 ± 12.6 µg NH2 per mg of sheets, trypsin was bound using combinatory chemistry of EDAC and sulfo-NHS. The amount (215 ± 29.8 µg protein/cm2) and activity (24.8 ± 2.7 I.U./cm2) of immobilized biocatalyst as well as storage and operational stability were measured. The proteolytic activity of immobilized trypsin determined immediately after immobilization and during one week was high and stable.Prepared bio-functional nanofiber material can find the application in wound healing process (enzyme debridement).
Keywords: inorganic nanofibers, electrospinning, trypsin, immobilization© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.