AMINE FUNCTIONALIZED CARBON NANOPARTICLES GRAFTED BIOPOLYMER FOR CELL ADHESION AND PROLIFERATION

1 ŽÁKOVÁ Pavlína
Co-authors:
1 SLEPIČKOVÁ KASÁLKOVÁ Nikola 1 SLEPIČKA Petr 1 ŠVORČÍK Václav
Institution:
1 University of Chemistry and Technology Prague, Department of Solid State Engineering, Prague, Czech Republic, EU, pavlina.zakova@vscht.cz
Conference:
7th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 14th - 16th 2015
Proceedings:
Proceedings 7th International Conference on Nanomaterials - Research & Application
Pages:
500-504
ISBN:
978-80-87294-59-8
ISSN:
2694-930X
Published:
11th January 2016
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
490 views / 201 downloads
Abstract

Various carbon nanostructures are widely researched for use in a number of medical applications. We study the surface properties and cell-substrate interactions of amine functionalized carbon nanoparticles (CNPs) grafted on biopolymer film. Poly-L-lactic acid, in form of polymer film, was treated in an inert argon plasma discharge and, subsequently, grafted with functionalized carbon nanoparticles. Selected samples were thermally stressed during or following the grafting procedure. The surface properties were studied using multiple methods (goniometry, X-ray photoelectron spectroscopy). Cell-substrate interactions were determined in vitro by studying adhesion, proliferation and viability of vascular smooth muscle cells (VSMCs) from the aorta of a rat. Cell-substrate interactions on pristine and modified substrates were compared to standard tissue culture polystyrene (TCPS). Our results show that CNPs affect surface morphology and wettability and therefore adhesion, proliferation and viability of cultured cells.

Keywords: Carbon nanoparticles, Polymer film, Surface plasma and thermal treatment, Surface properties, Cytocompatibility

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