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The paper describes an innovative method of determining cytotoxicity rate for non-transparent materials. Cytotoxicity is a very important property of materials in terms of their use in health care. It means that all materials with a targeted use in a field of interaction with tissue have to be tested for cytotoxicity. Therefore it is necessary to have a quick and reliable method of determination available. Innovative method for testing cytotoxicity of non-transparent materials is based on monitoring cell-material interaction directly on its surface. Important non-transparent materials used in medicine are titanium alloys. The method is using MG63 cell line which is inoculated on the surface of a material Titan Grade 2 (pure titanium), TiGr5 (Ti6Al4V) and TNT (Ti36Nb4Ta) and cultivated there for approximately three successive generations. The cultivated cells are fluorescently stained so that they are visible in the incident light. Afterwards an analysis of surface coverage of material by cells is accomplished by the means of semi-automatic segmentation software. Image recording ready to be applicable for further analysis is an advantage of this method. The innovative aspect of the method is in utilization of fluorescence for capturing images of cell colonization, of the surface and subsequent semi-automatic determination of the colonization surface which guarantees objective and reproducible results. The determined percentage of tolerance was 68.88 % for TiGr2, 74.01 % for TiGr5 and 77.46 % for TNT.
Keywords: Titan alloys, cytotoxicity, cell-alloy interaction, cell segmentation© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.